Evaluation of the Fluo-RAL module for detection of tuberculous and nontuberculous acid-fast Bacilli by fluorescence microscopy.
نویسندگان
چکیده
C. Bernard, C. Wichlacz, M. Rigoreau, S. Sorhouet, R. Dagiral, V. Jarlier, N. Veziris UPMC, Université Paris 06, Laboratoire de Bactériologie-Hygiène, Paris, France; AP-HP, Hôpital Pitié-Salpêtrière, Laboratoire de Bactériologie-Hygiène, Paris, France; Centre National de Référence des Mycobactéries et de la Résistance des Mycobactéries aux Antituberculeux, Paris, France; Recherche et Développement, RAL Diagnostics, Martillac, France
منابع مشابه
EVALUATION OF DIRECT MICROSCOPY, CULTURE, AND POLYMERASE CHAIN REACTION FOR THE DIAGNOSIS OF TUBERCULOUS MENINGITIS
In view of the importance of early diagnosis of tuberculous meningitis (TBM), the efficiency of the polymerase chain reaction (PCR), one of the most reliable and sensitive DNA-based assays, was compared with conventional methods (acid-fast microscopy and culture) for the detection of M. tuberculosis in cerebrospinal fluid (CSF) specimens from patients suspected of TBM. Of the 29 CSF specim...
متن کاملEvaluation of a fluorescence in situ hybridization assay for differentiation between tuberculous and nontuberculous Mycobacterium species in smears of Lowenstein-Jensen and Mycobacteria Growth Indicator Tube cultures using peptide nucleic acid probes.
A new fluorescence in situ hybridization assay based on peptide nucleic acid probes (MTB and NTM probes targeting tuberculous and nontuberculous species, respectively) for the identification of Mycobacterium tuberculosis complex and differentiation between tuberculous and nontuberculous mycobacteria (NTM) was evaluated using Lowenstein-Jensen (LJ) solid cultures from 100 consecutive sputum samp...
متن کاملSensitivity of fluorochrome microscopy for detection of Mycobacterium tuberculosis versus nontuberculous mycobacteria.
The results for 6,532 consecutive mycobacterial respiratory specimens collected from 1,040 patients from 1993 to 1995 in a Texas hospital were studied to determine the sensitivity of fluorescence microscopy for detection of Mycobacterium tuberculosis and nontuberculous mycobacteria (NTM). Smears were positive for acid-fast bacilli (AFB) in 63% (677 of 1,082) of specimens growing M. tuberculosis...
متن کاملTHE DETECTION OF Mycobacterium tuberculosis BY PCR IN SPUTUM SAMPLES FROM LONG-TERMTREATED LEPROSY PATIENTS: ASSOCIATIONS WITH SKIN TEST RESULTS AND IMMUNOTHERAPY WITH Mycobacterium vaccae
As part of a series of investigations at Baba Baghi Leprosarium in lran, 44 long-treated leprosy patients were selected for our study. Samples of early morning sputum were obtained from each patient, examined by microscopy for acid-fast bacilli (AFB), and cultured for tubercle bacilli. These tests were negative, but the polymerase chain reaction (PCR) for an insertion sequence believed to b...
متن کاملIncidental unilateral tuberculous sacroiliitis detected by 18F-FDG PET/CT in a patient with abdominal tuberculosis.
Tuberculosis is a systemic disease which involves skeletal and articular system very rarely. Osteoarticular tuberculosis commonly occurs in the vertebral column and more rarely in the sacroiliac joints. In this study, we report a 44-years-old male patient with low-grade fever, malabsorption syndrome, abdominal and pelvic ascites and low-back pain, that underwent 18F-FDG PET/CT for identifying t...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 51 10 شماره
صفحات -
تاریخ انتشار 2013